Molecular Diagnostic and Capacity Building Workshop on Influenza A H1N1/2009 virus and other respiratory diseases of Swine

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Tue, 01/31/2012 - 15:00 - Fri, 02/03/2012 - 15:00

The Sub-regional Coordinator of the Food and Agriculture Organization of the United Nations (FAO) in Barbados with the French Agricultural Research Centre for Development (CIRAD) organized an activity of the Project TCP/RLA/3306 (D): “Technical assistance for surveillance of Influenza A H1N1/2009 virus and other Influenza viruses in swine populations in the Caribbean region”. CIRAD Guadeloupe hosted a Workshop on the diagnosis of Influenza A H1N1/2009 virus and other viral respiratory diseases of swine in Petit Bourg, from 31st January to 3rd February 2012.

This FAO/CIRAD Co-funded workshop was a follow-up to the “Field Surveillance, Epidemiology and Risk Communication Workshop for Swine Influenza viruses in the Caribbean Region”, which was held in Port-of-Spain, Trinidad and Tobago in July, 2011.


Objectives of the training

The general objective of the molecular diagnostic and capacity building workshop was to train participants in molecular diagnostic techniques with particular focus on real time Reverse-Transcriptase Polymerase Chain Reaction (QRT-PCR). The workshop also trained the participants in the following areas: the fundamentals of veterinary diagnostic laboratory management; laboratory quality assurance principles including introduction to quality assurance and laboratory bio-safety procedures. The 4 days workshop provided the participants with the tools to:

•    Carry out analysis of samples using RT-PCR; perform RNA extraction protocol from swabs

•    Perform RT-PCR using a set of primers specific of H1N1/2009 Influenza virus and other primers in order to differentiate with seasonal H1N1 (WHO protocols and other protocols).

•    Prepare and submit samples for additional analysis to reference laboratories

•    Information on the diagnosis of other viral diseases of swine (CSF, Circovirus, PRRS, etc.)

•    Implement the fundamentals of veterinary diagnostic laboratory management and laboratory quality assurance principles.

The workshop included practical work in the laboratory and participants got hands-on experience in samples processing and PCR. There were also theoretical sessions in laboratory management and laboratory quality assurance programmes.




Participants list and the final program of the workshop are available bellow.



list_of_participants.pdf259.17 KB
agenda.pdf540.57 KB


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